Applied Bio Research Inc.

Working in harmony with nature...

Phage Technology

Overcoming barriers to optimize phage technology: Please contact us for further details.

Our phage reprogramming technology does not employ genetic manipulation or modification. It is used to culture phages with elevated virility for treating previously resistant bacterial strains. The technology is inexpensive and reliable, converting phages from lysogenic (dormant) to a lytic (active) state within the host organism.

This process allows us to formulate highly effective phage masters, isolated from the wild and able to kill > 95% of target bacteria. We also overcome the usual barriers to the effective use of phages in the control of bacteria and eliminate the need to return to a field source of bacteria to culture new phages. 
This technology enables us to develop innovative solutions for medical and pharmaceutical industries and includes using phage biotechnology to create environmentally friendly disinfectants, detergents, bio-decontamination, anti-biofilms, cosmetics and rapid diagnostic kits.

The following slides are excerpts from more detailed presentations on each technology:

Phage lytic cycle and lysin enzymes formation: (6 diagrams)

Some of the many novel uses for Applied Bio Research phage technologies:

Applications for Applied Bio Research phage technology:

Phages used as feed supplements enhance probiotic activity 

Probiotics are used in animal feed to repair the deficiencies in the microflora and modulate the balance and activities of the gastrointestinal microbiota to restore the animals' resistance to disease. However, antibiotics kill off the beneficial bacteria or probiotics along with bacterial pathogens. The reduction of beneficial bacteria can lead to digestive problems, such as antibiotic-associated diarrhea resulting from an imbalance in the colonic microbiota caused by antibiotic therapy. ABR has developed programmed phage that target only pathogenic bacteria. These biological antimicrobials, unlike antibiotics, are species specific and thus do not damage commensal bacteria. They improve the microflora of the farm animals by targeting only specific problem bacterial pathogens in the animal gut.

Many health benefits from animal intake of programmed phage with probiotics

  • Improve animal immune system

  • Increased growth rate

  • Improved feed digestion

  • Improved resistance to disease

  • Improved milk yield and quality

  • Improved egg production

Clean water from commercial-industrial grey water

Over one billion people do not have enough safe water to drink and thus will never live a healthy life. In developing nations, 90% of sewage and 70% of industrial waste is discharged untreated, into rivers, streams and coastal waters. Our PhytoPhage solutions can be applied to clean polluted water and for cleaning and recycling commercial and industrial wastewater streams. This technology is inexpensive, environmentally friendly and can dramatically reduce waterborne pathogens and carcinogenic chemicals (e.g. aluminum, fluoride and chlorine).

Put an end to cyanobacterial (blue-green algae) blooms for a healthy ecosystem

The incidence of cyanoHABs (cyanobacterial harmful algal blooms) globally are increasing despite many strict control measures that have been implemented to reduce the excessive level of phosphorus and fertilizers in aquatic environments. ABR has innovated a protocol that can rapidly detect cyanobacterial cells and it also effectively controls and reduces cyanoHABs in marine and freshwater environments. The system is based on using smart lytic cyanophages to help control the excess cyanobacteria which form the harmful blooms.

The power of CO2 - algae for biofuels:

Algae produces 30 times more energy per acre than corn, soybeans or sugarcane and can grow in salt water, our world’s most abundant resource. The advantages of deriving biodiesel from algae include rapid growth rates and a high per-acre yield. Algae biofuel also contains no sulphur, is non-toxic and is highly biodegradable. Some species of algae are ideally suited to biodiesel production due to their high lipid content, some with as high as 50% oil content. We can shorten the time to algae harvest and increase the lipid content, with up to 86% more neutral lipid in some species, by using a specific, environmentally friendly bacterium. We propose the recovery and re-utilization of industrial carbon dioxide and wastewater streams, which contain nutrients that create an excellent habitat for algae production and could also greatly reduce the carbon footprint of these industries.

Cyano-electrogenic technology: creating electricity, another great use for algae!

Cyanobacteria account for 20–30% of the earth's photosynthetic productivity. They’re able to convert sunlight directly into electricity in a COneutral manner. On a global scale,
the amount of solar energy that is harvested by cyanobacteria exceeds 25 times the energy consumed by humans. Unlike other photosynthetic prokaryotes, they utilise the energy
of sunlight to drive photosynthesis, H2O molecules split into O2, protons and electrons (e-) and a portion of hydrogen (H2). Less than 95% e- are utilised by the cyanobacterial cells
for their own needs and ≥5% of these e- are donated to the external environment ‘biosphere’.

Sunlight + CO2 + 2H2O   ----->      (CH2O) + H2O + O2 + e- + Protons + small amount of H2.

On a global scale, the average rate of e- discharge or transfer of solar energy to the environment via the cyanobacterial electrogenic pathway could exceed ~9 TW (terawatts). Harvesting the excess energy from this renewable resource makes good economic and environmental sense.

Website Builder